Sequence for human argininosuccinate synthetase cDNA
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چکیده
منابع مشابه
Cloning and sequence analysis of cDNA for human argininosuccinate lyase.
Using antibodies specific for argininosuccinate lyase (EC 4.3.2.1), we isolated two cDNA clones by screening a human liver cDNA library constructed in the lambda gt11 expression vector. The identity of these isolates was confirmed by in vitro translation of plasmid-selected mRNA. One of these isolates was used to rescreen the cDNA library and a 1565-base-pair (bp) clone was identified. The enti...
متن کاملNitro analogs of substrates for argininosuccinate synthetase and argininosuccinate lyase.
The nitro analogs of aspartate and argininosuccinate were synthesized and tested as substrates and inhibitors of argininosuccinate synthetase and argininosuccinate lyase, respectively. The Vmax for 3-nitro-2-aminopropionic acid was found to be 60% of the maximal rate of aspartate utilization in the reaction catalyzed by argininosuccinate synthetase. Only the nitronate form of this substrate, in...
متن کاملParadoxical regulation of human argininosuccinate synthetase cDNA minigene in opposition to endogenous gene: evidence for intragenic control sequences.
Human somatic cell variants resistant to the arginine analog, canavanine, express 200-fold increased levels of argininosuccinate synthetase (AS) mRNA as compared to parental cells. In this study we examined whether AS cDNA sequences contain cis-acting regulatory elements that are involved in the induction of AS mRNA in canavanine-resistant cells. Minigene constructs containing AS cDNA sequences...
متن کاملMolecular analysis of argininosuccinate synthetase deficiency in human fibroblasts.
We have analyzed cultured skin fibroblasts derived from patients with argininosuccinate synthetase deficiency for alterations in gene structure, mRNA content, and protein structure. Genomic DNA was digested with the endonucleases EcoRI or HindIII, and the fragments were analyzed by Southern blotting and hybridization with a cDNA probe for argininosuccinate synthetase. The blot pattern is comple...
متن کاملCloning of cDNA for argininosuccinate synthetase mRNA and study of enzyme overproduction in a human cell line.
Previous studies of the human cell line RPMI-2650 (wild type) and its canavanine-resistant variants have demonstrated differences in argininosuccinate synthetase activity as follows: canavanine-resistant much greater than wild type grown in citrulline greater than wild type grown in arginine (Su, T.-S., Beaudet, A. L., and O'Brien, W. E. (1981) Biochemistry 20, 2956-2960). A recombinant plasmid...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1983
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/11.18.6505